Green Options

Bacterial evolution can be accelerated with the MAGE technique to produce large numbers of  favorable mutations (micrograph image magnified 10, 000 times)

Bacteria are prolific replicators, and some species can replicate into the millions in just a few hours. Bacteria, in the functioning of their cellular and biochemical machinery, also just happen to manufacture some very useful chemicals and bio-active molecules. The microbe populations also exhibit high rates of random mutation, which can confer adaptive traits, over time, onto the newer, variant population.

These attributes of bacterial life forms have been exploited in the lab (and in other industries) for some time, but generating genomic diversity in the lab has been challenging; inserting genes or entire genetic sequences into a cell’s nucleus (and DNA) can be done readily, but controlling or directing how exactly these hybrids mutate, is quite another thing. Further, new phenotypes (the main physical traits or properties) don’t usually happen fast or frequently enough for practical uses. But with a new technique called MAGE (Multiplex Automated Genome Engineering), bacteria are now being engineered (and “directed”) to perform these functions much faster and much more efficiently.

Scientific American reports that like so many elements in the world, it’s all in the use and volume for whether that something, say poison or E. coli, is a friend or foe: “Escherichia coli (E. coli) can give you a severe case of food poisoning or, with a little genetic engineering, a useful plastic.”

San Diego-based scientists at Genomatica have developed the ability to manipulate bacteria into being useful to feed our societal lust for plastics, by producing “butanediol (BDO), a chemical compound used to make everything from spandex to car bumpers, thereby providing a more energy-efficient way of making it without oil or natural gas,” the article says.